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學術報告(20190531)Studies on the dynamics and regulation of 30-nm chromatin fiber by single molecule force spectroscopy
發布時間:2019-05-28

報告題目: Studies on the dynamics and regulation of 30-nm chromatin fiber by single molecule force spectroscopy
報告人: 李偉 副研究員
報告人單位: 中科院物理所
報告時間: 5月31日(周五)上午10點
報告地點: 科技樓北410
報告人簡介:

李偉,中科院物理所副研究員,博士生導師。2006年獲得中科院物理所博士學位亚博娱乐登录网址。目前亚博娱乐登录网址,主要從事生物物理交叉學科的研究,利用磁鑷單分子力譜技術,并結合冷凍電鏡、原子力顯微鏡等手段,跟蹤生物大分子(DNA和蛋白質)的動態結構,分析其在生命活動中的分子機制及功能亚博娱乐登录网址。在JACS、 PRL、Molecular Cell、Genes & Development亚博娱乐登录网址、PRE等刊物上發表學術論文30余篇。最近在染色質動態結構和調控方面取得一系列進展,相關工作被引述為Nature Reviews封面亚博娱乐登录网址。2017獲得中科院前沿重點項目資助,2018年獲得北京市科學技術進步二等獎。
報告摘要:

In eukaryotes, the packaging of genomic DNA into chromatin plays a critical role in gene regulation. However, the dynamic organization of chromatin fibers and its regulatory mechanisms remain poorly understood. Using single molecule magnetic tweezers, we reveal that the tetranucleosomes-on-a-string appears as a stable secondary structure which is attenuated by histone chaperone FACT. Furthermore, we reveal that FACT’s subunit SSRP1 is responsible for maintenance of the nucleosome integrity by holding H3/H4 tetramer on DNA. In contrast, the large subunit SPT16 destabilizes the nucleosome structure by displacing H2A/H2B dimers. Our findings provide mechanistic insights by which the two subunits of FACT coordinate with each other to fulfill its functions. To trace the details of chromatin folding and unfolding dynamics and to reveal the spatial relationship between DNA and histones, we are developing a new method of combing single molecule magnetic tweezers and single molecule   FRET.


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